Fig 1. Mycobacterial growth under conditions of acidic pH and high salt concentration promote phosphorylation of PhoR and PhoP.

Phos-tag analysis of cell lysates of WT-H37Rv harbouring His-tagged (A) PhoR, (B) PhoP or (C) phosphorylation-defective PhoPD71N proteins, were grown either under normal conditions (pH 7.0) or under indicated stress conditions, as described in the Methods. PhoR and PhoP were detected by Western blotting using anti-His antibodies, and RpoB (as a loading control) was detected in cell lysates using anti-RpoB antibody (Abcam). Data are representative of two independent experiments.