Video 5. Fluorescence and phase-contrast time-lapse video of a human RPE1 cell stably expressing eGFP-nucleolin after flavopiridol washout.
The cell was pretreated with 10 µM flavopiridol for 5 hr, then the drug was washed out before imaging. Nucleoli re-form within 4–6 hr. Time is indicated as minutes after drug washout and initiation of imaging. Bar, 10 μm.