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. 2023 Dec 15;14:8362. doi: 10.1038/s41467-023-43602-7

Fig. 4. Loss of top-ranked neuron-essential TFs disrupts neurogenesis.

Fig. 4

a Targeting of individual neuron-essential TFs in human embryonic stem cells to quantify MAP2-negative cell numbers. b Flow cytometry of MAP2-tdTomato expression at 7 days after NEUROG1/2-induction in cells transduced with Cas9 and guide RNAs targeting ZBTB18 (3 distinct guide RNAs), tdTomato (1 representative guide RNA), or a non-targeting (negative) control (1 representative guide RNA). c Change in MAP2-negative cells (compared to non-targeting control) when targeting the indicated gene (n = 3 guide RNAs per gene).