Skip to main content
. 2023 Dec 15;14:8369. doi: 10.1038/s41467-023-43380-2

Fig. 8. ERG11 gene copy number increasing induced in vitro results in enhanced azole resistance.

Fig. 8

a Design of the in vitro induction experiment. Cluster AZR strain ID no. F4082 (ERG11 copy number = 3, fluconazole MIC = 128 mg/L) was selected and passaged 10 times in vitro under fluconazole stress of 256 mg/L. The 0th, 3rd, 6th, and 10th passages were specifically selected to evaluate alterations in antifungal susceptibility, ERG11 expression level, and ERG11 CNVs. b At the 10th passage, the ERG11 gene copy number increased to n = 7. c A 2-fold increase in fluconazole MIC was observed at the 10th passage. d Expression level of the ERG11 gene also showed a 2-fold increase; n = 3 independent experiments were carried out, and data was presented as mean values ± SD.