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. 2023 Dec 16;14(12):834. doi: 10.1038/s41419-023-06350-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Density plots and gating strategy from single-cell suspensions of live CD45 CD11b-infiltrating cells at baseline, days 1 and day 7 after retinal detachment. B Histogram overlays of CD45^hi CD11b⁺ Ly6G⁺ and CD45^hi CD11b⁺ Ly6G⁻ Ly6C⁺ cells. C–E Quantitative analyses of total CD11b⁺, CD45^hi CD11b⁺ Ly6G⁺, and CD45^hi CD11b⁺ Ly6G⁻ Ly6C⁺(pooled samples, n = 6 per replicate). F Quantitation of subretinal cells in the subretinal space of whole-eye cryosection images (n = 4 per group). Images are representative of ≥2 experiments. G and H Representative retinal cryosection image of subretinal CD11b⁺ cells at day 1 and 14, showing cell size increase (n = 4, 4). I Subretinal CD11b⁺ cell size ratio thorough days 1–14 after detachment. J Ratio of CD11b⁺ >100 µm² area. K Location of subretinal CD11b⁺ in the subretinal space (SRS) or in contact with the outer nuclear layer (ONL) (n = 4 per group). *p < 0.05.