FIG. 1.

Immunoblotting of FLAG-containing proteins in BD170-VB B. subtilis cells overexpressing different Bmr variants. Bmr was either unmodified (lane 1), FLAG modified (lane 2), FLAG-modified P347T mutant (lane 3), FLAG-modified F143V mutant (lane 4), or FLAG-modified F143V P347S double mutant (lane 5). Forty micrograms of total cellular protein was loaded in each lane. The apparent molecular mass of Bmr is 35 kDa, which is lower than the theoretically predicted 42 kDa, likely due to the high hydrophobicity of this protein. Sizes are indicated in kilodaltons.