Fig. 3.

Immune phenotype of patient, mother, and two healthy controls (HC) by flow cytometry. Markers used for staining are indicated on the arrows next to the contour plots, gated populations above. For gating strategy, see Fig. S4. A (upper row) CD4 vs. CD8 expression on CD3⁺ lymphocytes, CD4/CD8 ratio in red; (lower row) αβ and γδT-cell frequency in CD3⁺ lymphocytes. B CD4⁺ T-cell differentiation (upper row) and CD57 expression (lower row). C CD8⁺ T-cell differentiation (upper row) and CD57 expression (lower row). D CD4⁺ CD45RO⁺ CCR6^- T helper cell phenotype. F Treg cells