TABLE 1.

Bacterial strains and plasmids used

Strain or plasmid	Genotype or construction	Source or reference
E. coli
HB101	subE44 subF58 hsdS3(rB−mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1	6
WM2015	subE thi Δ(lac-pro)	30
WM2017	WM2015 himA::TcrhimD::Cmr	30
P. putida
PaW85	Tn4652	4
PRS2000	Tn4652-free	45
KT2442	Tn4652 xylRS Pu-lacZ Rifr Smr	8
A8759	KT2442 Tn4652 ihfA::Kmr Pu-lacZ	8
Plasmids
pBluescript KS	Cloning vector (Apr)	Stratagene
pEST1332	Plasmid pAYC32 carrying promoterless pheBA operon	27
p1332S/C	PCR-generated 122-bp Tn4652 right-end fragment (primers Osac and Ocla) cloned into pEST1332	This work (Fig. 2)
p1332IHF/C	PCR-generated 83-bp Tn4652 right-end fragment (primers Oihf and Ocla) cloned into pEST1332	This work (Fig. 2)
p1332D/C	SacI-DraI deletant of p1332S/C	This work (Fig. 2)
p1332S/N	NheI-ClaI deletant of p1332S/C	This work (Fig. 2)
pKT240	Cloning vector (Apr Kmr)	3
pKRZ-1	Cloning vector (Apr Kmr)	37
pKTlacZ	Promoter probe vector containing lacZ gene from pKRZ-1 cloned into pKT240	This work (Fig. 1)
pKTlacZS/C	PCR-generated 122-bp Tn4652 right-end fragment (primers Osac and Ocla) cloned into pKTlacZ	This work (Fig. 2)
pKTlacZIHF/C	PCR-generated 83-bp Tn4652 right-end fragment (primers Oihf and Ocla) cloned into pKTlacZ	This work (Fig. 2)
pKTlacZD/C	SacI-DraI deletant of pKTlacZS/C	This work (Fig. 2)
pHNβα	Plasmid carrying E. coli IHF genes ihfA and ihfB	31
pUC18	Cloning vector (Apr)	48
pUCPu130	129-bp DpnI fragment of Pu promoter region of xyl genes in TOL plasmid cloned into pUC18	This work