Fig. 1.

Effects of DHA on cell death in the LPS-induced HL-1 cardiac cells. A) Cell viability was scored by methyl thiazolyl tetrazolium (MTT) assay. B) LDH content in the cell supernatant was utilized as a surrogate for cell death and quantified using Cytotoxicity Detection Kit. C) Caspase 3 activity was used to analyze apoptosis. D) The expression level of Cleaved caspase-3 was assayed by Western blot. n = 3 independent experiments. *P<0.05, **P<0.01, compared with the control group; ^#P<0.05, ^##P<0.01compared with the LPS group.