TABLE 3.

Conversion of 3-chloro-2-hydroxymuconic semialdehyde by cell extracts from P. putida mt-2^a

Substrate (initial concn, 60 μM)	Sp act (μmol min−1 mg of protein−1)
	No supple- ments	+NADase	+NAD+
2-Hydroxymuconic semialdehyde (ring fission product of catechol)	0.27	0.22	0.49
2-Hydroxy-6-oxo-hepta-2,4-dienoate (ring fission product of 3-methylcatechol)	2.24	2.24	2.24
3-Chloro-2-hydroxymuconic semialdehyde (ring fission product of 3-chlorocatechol)	0.0007	0.0006	0.0007

^aCells were grown with 3-methylbenzoate at 30°C. Experimental details are given in Materials and Methods.