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. Author manuscript; available in PMC: 2023 Dec 16.
Published in final edited form as: Immunopharmacol Immunotoxicol. 2019 Jun 2;41(3):361–369. doi: 10.1080/08923973.2019.1616754

Figure 1. Sorting FLT3-L DC culture by positive selection of pDCs using surface expression of B220.

Figure 1.

pDCs and cDCs were generated in vitro from bone marrow precursor cells harvested from the femur and tibia of WT mice in FLT3-L enriched-medium. After 7 days in culture, cells were gently harvested, and pDCs were positively selected for surface expression of B220, a CD45 isoform expressed by murine pDCs but not cDCs. The positively selected cohort of cells for all experiments displayed a purity of at least 90%. Flow diagrams are representative of purity checks for all the experiments in which pDCs were sorted by magnetic beads, showing the expression of CD11c and B220 before sorting (left) and in the positively selected pDC subset (center) and cDC subset (right).