Fig. 1.

Quantitative analysis of PDE4D, HCN1, and mGluR3 localization in macaque ERC layer II circuits. (A) The prevalence of PDE4D in various cellular subcompartments in layer II of the ERC neuropil, expressed as percentage of PDE4D profile (e.g. dendrite) per total PDE4D profiles. PDE4D is primarily expressed in postsynaptic subcompartments in ERC layer II microcircuits, with foremost expression in dendritic shafts, and significant expression within dendritic spines and astroglia. (B) The prevalence of HCN1 in various cellular subcompartments in layer II of the ERC neuropil, expressed as percentage of HCN1 profile (e.g. dendritic spine) per total HCN1 profiles. HCN1 is primarily expressed in postsynaptic subcompartments in ERC layer II microcircuits, with greatest expression in dendritic spines, and significant expression within dendritic shafts. (C) The prevalence of mGluR3 in various cellular subcompartments in layer II of the ERC neuropil, expressed as percentage of mGluR3 profile (e.g. glia) per total mGluR3 profiles. mGluR3 is predominantly expressed in glia, with significant expression in postsynaptic subcompartments in ERC layer II microcircuits, including in dendritic spines and dendritic shafts. For additional details regarding quantitative assessment and profile identification, see the “Materials and Methods” section. Nondetermined (N.D) are profiles that could not be unequivocally categorized.