Table 3.
Differentially expressed genes in CD4+T cell subsets
| Gene ID | Gene name | Fold change |
Function/gene ontology |
|---|---|---|---|
| (a) Microarray analyses of downregulated CD4+T cell genes from pCons-tolerized vs. naïve mice | |||
| Zfp146 | Zinc finger protein 146 | −24.3 | DNA binding/regulation of transcription |
| 9230110C19Rik | RIKEN cDNA 9230110C19 gene | −17.1 | Unknown |
| Wbscr1 | Williams–Beuren syndrome chromosome region 1 homolog | −17.1 | RNA binding/translation initiation factor activity |
| Dixdc1 | DIX domain containing 1 | −14.9 | Actin binding/signal transduction |
| - | 9 days embryo whole body cDNA, RIKEN library | −13.0 | Unknown |
| - | cyclin D binding myb-like transcription factor 1 | −12.1 | DNA binding/regulation of transcription/cell cycle regulation |
| - | nuclear receptor subfamily 2, group C, member 2 | −9.8 | DNA binding/regulation of transcription/signal transduction |
| 2210010C04Rik | RIKEN cDNA 2210010C04 gene | −8.6 | Serine-type peptidase activity/proteolysis |
| Mfhas1 | Predicted: similar to malignant fibrous histiocytoma amplified sequence 1 | −8.6 | Protein binding |
| - | RIKEN cDNA 1300007C21 gene | −5.7 | Unknown |
| Hrb | HIV-1 Rev-binding protein | −5.7 | Regulation of GTPase activity/macromolecular localization/spermatogenesis |
| - | ESTs | −5.7 | Unknown |
| Amy1 | Amylase 1, salivary | −4.9 | Hydrolase activity/carbohydrate metabolism |
| 9030625G08Rik | RIKEN cDNA 9030625G08 gene | −4.9 | DNA binding/Zinc ion binding/metal ion binding |
| 2210401K01Rik | Adult male stomach cDNA, RIKEN library, receptor (calcitonin) activity | −4.6 | Unknown |
| Pnlip | Pancreatic lipase | −4.6 | Lipid/sterol metabolism |
| Zfhx1b | Zinc finger homeobox 1b | −4.3 | DNA binding/regulation of transcription/cell migration |
| Igh-VJ558 | Clone X1AC2145 immunoglobulin heavy chain variable region | −4.3 | antigen binding/immune response |
| 1700012F10Rik | WD repeat domain 68 | −4.3 | Signal transduction |
| Rbm5 | RNA binding motif protein 5a | −4.3 | RNA binding |
| Peli1 | Pellino 1 | −4.3 | Ubiquitin-protein ligase activity/scaffold protein |
| Ela3b | Elastase 3B, pancreatic | −3.7 | Serine-type endopeptidase activity/proteolysis |
| Trip12 | Thyroid hormone receptor interactor 12 | −3.7 | Ubiquitin-protein ligase activity/protein metabolism/signal transduction |
| - | ESTs | −3.7 | Unknown |
| 2210401K01Rik | Adult male stomach cDNA, RIKEN library | −3.5 | Unknown |
| Luc7l2 | LUC7-like 2 | −3.5 | Development |
| - | RIKEN cDNA 9030402K04 gene | −3.5 | Unknown |
| D12Ertd771e | DNA segment, Chr 12, ERATO Doi 771, expressed | −3.5 | Unknown |
| 9030612M13Rik | MKIAA3006 protein | −3.2 | Nucleic acid binding/regulation of transcription |
| Ddx6 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 | −3.0 | ATPase activity/RNA binding/RNA helicase activity |
| Akt1 | Thymoma viral proto-oncogene 1 | −3.0 | Protein kinase activity/signal transduction/Anti-apoptosis/cell cycle regulation |
| 1110014N23Rik | Predicted: hypothetical protein LOC68505 | −3.0 | Unknown |
| - | ESTs | −3.0 | Unknown |
| Sec61a1 | Sec61 α-1 subunit | −2.8 | Protein translocase activity/protein secretion |
| Snrpd3 | Small nuclear ribonucleoprotein D3 | −2.8 | RNA splicing |
| Tcf12 | Transcription factor 12 | −2.8 | DNA binding/regulation of transcription |
| D12Ertd551e | DNA segment, Chr 12, ERATO Doi 551, expressed (D12Ertd551e) | −2.8 | Unknown |
| Ddx50 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 | −2.8 | ATPase activity/RNA binding |
| (b) Microarray analyses of upregulated CD4+ T cell genes from pCons-tolerized vs. naive mice | |||
| Acly | ATP citrate lyase | 18.4 | ATP citrate synthase activity/Acetyl-CoA biosynthesis/lipid metabolism |
| Vrk2 | Vaccinia-related kinase 2 | 18.4 | Protein kinase activity |
| Ocil | C-type lectin domain family 2, member d (Clec2d) | 8.0 | Transmembrane receptor activity/signal transduction/regulation of myeloid differentiation, immune cell-mediated cytotoxicity, osteoclast differentiation |
| - | ESTs | 5.7 | Unknown |
| Bbx | HMG-box transcription factor BBX, alternatively spliced | 4.6 | DNA binding/regulation of transcription |
| Thsd2 | Predicted: Thrombospondin, type I, domain 2 | 3.5 | Iron ion binding/electron transporter activity |
| Tacr2 | Tachykinin receptor 2 | 3.2 | Neuropeptide receptor activity/signal transduction |
| Hsp105 | Heat-shock protein 105a | 3.2 | Chaperone cofactor dependent protein folding/response to stress |
| Riok3 | RIO kinase 3 | 3.2 | Protein kinase activity |
Differentially expressed genes in tolerized vs naive BWF1 CD4+ T cells were obtained after microarray analyses. Fluorescence intensity and signal to log ratios were obtained as described in the Materials and methods section, and fold changes and P-values were determined.
a
Replicate probe sets.