Table 4.
Differentially expressed genes in CD8+ T cell subsets
| Gene ID | Gene name | Fold change |
Function/gene ontology |
|---|---|---|---|
| (a) Microarray analyses of downregulated CD8+ T cell genes from pCons-tolerized vs naive mice | |||
| Igl-V1 | Immunoglobulin lambda chain (IgL)a | −8.0 | Endogenous antigen binding and processing/MHC class I receptor activity/immune response |
| - | IgG light chain gene, V region | −6.1 | Antigen binding/immune response |
| - | RIKEN full-length enriched library, clone:A430018E01 | −5.7 | Unknown |
| Pde4b | Phosphodiesterase 4B | −5.3 | cAMP-specific phosphodiesterase activity/regulation of PKA signal transduction |
| Pde7a | Phosphodiesterase 7A | −5.3 | cAMP-specific phosphodiesterase activity/regulation of PKA signal transduction |
| Lasp1 | LIM and SH3 protein 1 | −4.6 | Actin binding/cytoskeletal organization/focal adhesion-mediated signal transduction |
| Pabpc1 | Poly A-binding protein, cytoplasmic 1 | −4.0 | RNA binding/RNA stability/translation |
| Smc4l1 | SMC4 structural maintenance of chromosomes 4-like 1 | −3.7 | ATPase activity/mitosis/sister chromatid exchange |
| Igh-VJ558 | Immunoglobulin heavy chain (J558 family) | −3.5 | Antigen binding/immune response |
| Capza1 | Capping protein (actin filament) muscle Z-line, α-1 | −3.0 | Barbed-end actin filament capping/negative regulation of actin filament depolymerization |
| Dnaja2 | DnaJ (Hsp40) homolog, subfamily A, member 2 | −2.8 | HSP70 co-chaperone/protein folding/protein transport |
| - | ESTs | −2.8 | Unknown |
| Mcl1 | Myeloid cell leukemia sequence 1 | −2.8 | Regulation of programmed cell death |
| Sesn3 | Sestrin 3 | −2.5 | Negative regulation of cell cycle progression |
| Arid4b | AT-rich interactive domain 4B (Rbp1 like) | −2.5 | Possible involvement in repression of transcription or RNA processing/splicing |
| Igtp | Interferon-inducible GTPase 2 | −2.5 | GTPase activity/Response to pest, pathogen or parasite |
| - | Mus musculus expressed sequence AI893585 | −2.3 | Unknown |
| Utrn | Utrophin | −2.3 | Cytoskeletal binding/calcium ion binding/muscle contraction/chemotaxis |
| Igl-V1 | IgL variable regiona | −2.3 | Endogenous antigen binding and processing/MHC class I receptor activity/immune response |
| Igh-VJ558 | Immunoglobulin heavy chain (J558 family) | −1.5 | Antigen binding/immune response |
| (b) Microarray analyses of upregulated CD8+ T cell genes from pCons-tolerized vs naive mice | |||
| S100a8 | S100 calcium-binding protein A8 (calgranulin A) | 3.2 | Calcium ion binding/chemotaxis/cellular locomotion/inflammation |
| H2-K1 | MHC class I heavy chain precursor (H-2K(d)) | 3.2 | Antigen binding and processing/MHC class I protein complex/immune response |
| Gypa | Glycophorin A | 3.2 | Cell adhesion molecule/cytoskeletal anchoring/response to osmotic stress |
| Prdx2 | Peroxiredoxin 2 | 2.8 | Thioredoxin peroxidase activity/response to oxidative stress/anti-apoptosis |
| S100a9 | S100 calcium-binding protein A9 (calgranulin B) | 2.8 | Calcium ion binding/chemotaxis/cellular locomotion/inflammation |
| Rsad2 | Viperin (Vig1)a | 2.6 | Interferon-inducible anti-viral activity |
| Alas2 | Aminolevulinic acid synthase 2 | 2.6 | N-succinyltransferase activity/porphyrin, heme biosynthesis |
| Cd24a | CD24a antigena | 2.5 | Cell surface antigen/regulation of T-cell expansion |
| 5730469M10Rik | RIKEN cDNA 5730469M10 gene | 2.5 | Unknown |
| Eraf | Erythroid-associated factor | 2.5 | Hemoglobin binding/hemopoiesis |
| Car2 | Carbonic anhydrase 2 | 2.3 | carbon-oxygen lyase activity/interconverts carbon dioxide and carbonic acid/respiration |
| 1110063G11Rik | MKIAA0481 proteina | 2.3 | Integral membrane protein |
| Hba-a1 | Hemoglobin-α, adult chain 1a | 2.1 | heme, iron, oxygen binding/oxygen transport |
| Slc4a1 | Solute carrier family 4 (anion exchanger), member 1 | 2.1 | Inorganic anion transport/intrinsic to plasma membrane |
| Mscp | Solute carrier family 25, member 37 (Slc25a37), variant 1 | 1.9 | Ion and metabolite exchange/mitochondrial transport |
| Alad | Aminolevulinate, delta-, dehydratase | 1.7 | Carbon-oxygen lyase activity/porphyrin, heme biosynthesis |
| Klf1 | Kruppel-like factor 1 | 1.7 | DNA binding/regulation of transcription/chromatin remodeling |
| Hbb-b1 | Hemoglobin, β-adult major chain | 1.6 | Heme, iron, oxygen binding/oxygen transport/respiration |
| Bpgm | 2,3-bisphosphoglycerate mutase | 1.4 | Bisphosphoglycerate phosphatase activity/glucose metabolism |
Differentially expressed genes in tolerized vs naive BWF1 CD8+T cells were obtained after microarray analyses. Fluorescence intensity and signal to log ratios were obtained as described in the Materials and methods section, and fold changes and P-values were determined.
a
Replicate probe sets.