Figure 5.

Molecular phylogenetic analysis of the ASICs by the neighbor-joining method and their domain architectures. (A) The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The evolutionary distances were computed using the Poisson correction method. Evolutionary analyses were conducted in MEGA7. (B) Domain architectures of ASICs from H. sapiens to A. queenslandica. ASC: The apical membrane of many tight epithelia contains sodium channels that are primarily characterized by their high affinity to the diuretic blocker amiloride.