FIG. 1.

Determination of nucleosides from S. solfataricus 16S rRNA (75°C culture) by LC-MS analysis of an enzymatic digest, using UV detection at 260 nm. Nucleoside identities established from chromatographic retention times and mass spectra: 1, ψ; 2, C; 3, U; 4, m⁵C; 5, Cm; 6, G; 7, Um; 8, Gm; 9, m²G; 10, ac⁴C; 11, A; 12, Am; 13, m⁶A; 14, m₂⁶A. Unnumbered peaks were shown by corresponding mass spectra not to represent nucleosides.