TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| P. putida | ||
| KT2440 | hsdMR Ben+ | 9 |
| KT2440-rpoN | KT2440, rpoN::Km | 20 |
| KT2440-IHF3 | KT2440, ihfA::Km | This study |
| E. coli | ||
| CC118 pir | recA pir lysogen | 25 |
| HB101 | recA Smr | 4 |
| Plasmids | ||
| pRJS1 | ihfA::Km at pEX100T | 6 |
| pRK600 | ColE1, RK2-Mob+, RK2-Tra+, Cmr | This laboratory |
| pUNφ19 | pUC19 derivative, pRP4 Mob+, Apr | This study |
| pUNxylR::Ω | pUNφ19 derivative bearing xyl1::Ω, Apr, Smr | This study |
| pWW0 | Tol+, xyl1+, xyl1+ | 35 |
| pWWΩXylR | pWW0::xylR::ΩSm | This study |
a
Abbreviations: Ben+ and Tol+, degradation of benzoate and toluene, respectively; Rifr, Smr, Apr, and Kmr, resistance to rifampin, streptomycin, ampicillin, and kanamicin, respectively.