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. 2023 Dec 16;13:22403. doi: 10.1038/s41598-023-49713-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DMP1-Cre/Ai9 labeled cells in thymus. (A,B) Frozen histology of 8 week old DMP1-Cre/Ai9 thymus for tdT (red), DAPI (blue) and (B) CD31 staining (green). White box indicates magnified area. (C) 10 day old thymus stained for CD3 (green) (D) Flow cytometric of single cell suspensions of thymus from 4 month old Cre− and Cre+ animals. Cell gate shown for CD45⁺tdT⁺ cells. (E) FACs plots of CD45⁺tdT⁺ bone marrow and thymus cells for CD11b and F4/80 and (F) CD31 and CXCR4 expression. (G) Flow cytometric analysis of tdT⁺ cells in bone marrow and thymus gated from CD45⁺CD11b⁺ cells. (H) Flow cytometric analysis of tdT⁺ cells in bone marrow and thymus gated from CD45⁺F4/80⁺ cells.