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. 2023 Dec 16;13:22403. doi: 10.1038/s41598-023-49713-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage properties of DMP1-Cre/Ai9 labeled hematopoietic fraction in bone marrow. (A) Bone marrow flush incubated with 1 mM latex YG beads and then stained for CD11b and F4/80. FACs plots are first gated on CD45⁺tdT⁺ population, then fluorescent latex beads. (B) Flow analysis of 12 week old mice quantified for the CD45⁺tdT⁺ population in mice treated for 3 h with 25 mg LPS, ***p = 0.0009. (C) Percent tdT⁺ cells in 3 month old control (Ctrl) and 6 days post lethal gamma irradiation (γIr) dose (950 rads) *p = 0.0327 (D–F) 8 week old DMP1-Cre/Ai9 animals 36 h after macrophage depletion with clodronate loaded liposomes compared to control. Representative flow cytometry analysis of the macrophage (MΦ) tdT⁺CD11b⁺ population for F4/80 and Ly6G which is quantified in (E) for percent Ly6G⁻F4/80⁺ MΦ cells, **p = 0.002 and (F) percent Ly6G⁺F4/80⁺ MΦ cells, ***p = 0.0001. (G–I) 8 week old DMP1-Cre/Ai9 animals 4 days after single dose of 5-FU. Representative flow cytometry analysis of macrophages within the tdT⁺CD11b⁺ population for F4/80 and Ly6G which is quantified in (H) for percent Ly6G⁻F4/80⁺ MΦ cells, **p = 0.0018, and (I) percent Ly6G⁺F4/80⁺ MΦ cells, **p = 0.0016. n = 3–4 and SEM is shown in all graphs.