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. 2023 Dec 16;13:22403. doi: 10.1038/s41598-023-49713-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DMP1-Cre/Ai9 labeled myeloid cells are enriched in erythroid islands and express erythroid island macrophage (EIM) markers. (A–C) BM from 8 week old DMP1-Cre/Ai9 animals were processed to obtain and analyze intact blood islands by flow cytometry, n = 3 (A) SSC-A versus SSC-H identifies Singlets and Multiplets which were gated and analyzed in the context of (B) CD45 and tdT. (C) Blood islands were further identified by flow cytometry as F4/80⁺Ter119⁺Multiplet events and EIM by additional markers Ly6G, CD11b, CD51, VCAM1, CD169, and ER-HR3. As a control, total Singlets and Multiplets are shown for these markers. Enrichment of blood islands and EIM markers occurs within CD45⁺tdT⁺Multiplets gates.