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. 2023 Dec 16;13:22403. doi: 10.1038/s41598-023-49713-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of G-CSF on the DMP1-Cre/Ai9 labeled erythroid blood islands and EIMs. G-SCF was administered to 5 week old DMP1-Cre/Ai9 animals at 50 mg/kg, twice a day for 3 days. BM was processed for intact blood islands, n = 3. (A) Quantified flow data of percent tdT⁺Multiplets in PBS and G-CSF treated mice, *p = 0.0218. (B) Representative FACs plots of tdT⁺CD11b⁺Multiplets with cells double positive for F4/80 and Ter119 which is quantified in (C) ***p = < 0.0001. (D) Histograms of G-CSF (black line) and PBS (grey filled line) treated mice for expression of EIM markers within the tdT⁺CD11b⁺Ter119⁺F4/80⁺Multiplet gates. (E–I) BM flushes with niche disruption and RBC lysis after G-CSF treatment. (E) Quantified flow data of percent tdT⁺Singlets in PBS and G-CSF treated mice. (F) Representative FACs plots of tdT⁺CD11b⁺F4/80⁺Ly6G⁻Singlets with cells double positive for CD169 and VCAM1 which is quantified in (G) **p = 0.0043. (H) Representative FACs plots of tdT⁺CD11b⁺F4/80⁺Ly6⁺Singlets which is quantified in (I) ***p < 0.0001. SEM is shown in all graphs.