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. 2023 Dec 16;13:22403. doi: 10.1038/s41598-023-49713-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Endogenous DMP1 expression and lineage tracing of DMP1 in bone marrow. (A,B) Femoral frozen sections from DMP-Cre/Ai9 (red) stained with anti-DMP1 antibody (cyan) in bone marrow (A) and cortical bone as positive control (B). White arrows indicate colocalization of DMP1-Cre labeled macrophages with DMP1 protein. Yellow arrows indicate DMP1-Cre labeled stromal cells with colocalization of DMP1 protein or within close proximity to secreted DMP1 protein. (C) Secondary only staining in a Cre− littermate as negative control. Blue-DAPI (D) Relative mRNA expression of DMP-1 from sorted tdT⁻ and tdT⁺ bone marrow cells. RNA from bone was used as positive control. Representative experiment shown, n = 2. (E) Quantification of CD45⁺tdT⁺ cells by flow cytometry in lineage traced 6 week old Tamoxifen injected iDMP1-Cre/Ai9 animals, n = 3–5. (F) Quantification of percent CD11b⁺F4/80⁺ that are CD45⁺tdT⁺ cells in lineage traced iDMP1-Cre/Ai9 animals, n = 3–4. SEM is shown in graphs.