TABLE 4.
Frequency of recombinants after H. pylori matings in 24-well tissue culture plates
| Strain(s)a | Mating conditions
|
Specr Strepr CFUb
|
Relative %c | ||
|---|---|---|---|---|---|
| Membrane (0.1-μm pore size) | Treatment with DNase (285 μg/ml) | No. | No./107 bacterial pairs | ||
| A alone | − | − | 0 | 0 | 0 |
| B alone | − | − | 0 | 0 | 0 |
| A + B | − | − | 102 ± 13 | 22 ± 4 | 100 |
| A + B | − | + | 5.8 ± 2.9 | 1.3 ± 0.4 | 6d |
| A + B | + | − | 68 ± 4 | 16 ± 5 | 70e |
| A + B | + | + | 0 | 0 | 0 |
a
Strain A = HPK1 (Specr Rifr); strain B = HPK5 (Strepr pHP1 Kanr).
b
Mean ± SEM from three replicate experiments.
c
Relative to A + B, no membrane, no DNase.
d
P < 0.05 in comparison with A + B, no membrane or DNase (Mann-Whitney test).
e
P > 0.05 in comparison with A + B, no membrane or DNase (Mann-Whitney test).