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. 2023 Dec 15;32:09636897231219830. doi: 10.1177/09636897231219830

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Characteristics of RM cells and growth morphology in 3D cultural system. (A) Isolation of RM cells from antler growth center. (B) Immunofluorescence staining using CD73, CD90, NESTIN, and SOX2. (C) Chondrogenic, osteogenic, and adipogenic induction using OriCell Mesenchymal Stem Cells Differentiation Kit (GUXMX-90041, GUXMX-90021, and GUXMX-90031, respectively). (D) Transferring RM cells into 3D cultural system (hollow fiber cell culture system), cell mass with different sizes were observed on the hollow fiber, histological sections, and Alcian Blue staining indicated that the cell mass were aggregated RM cells. RM: reserve mesenchymal; DAPI: 4′,6-diamidino-2-phenylindole.