FIG. 1.
Chitinase activity induced by colloidal chitin in the presence or absence of glucose in S. lividans TK24 and G015 (A); in TK24(pXE4), G015(pXE4), and G015(pGA01) (B); and in TK24(pGAH01) (C). Chitinase activity was measured with 4MU-(GlcNAc)2 for panel A and 4MU-(GlcNAc)3 for panels B and C. At time zero, cultures grown in LB medium at 30°C for 48 h were divided into three aliquots, and the mycelia in each aliquot were suspended in YE medium (open triangles), YE medium plus 0.05% (wt/vol) colloidal chitin (open circles), or YE medium plus 0.05% (wt/vol) colloidal chitin plus 1.0% (wt/vol) glucose (solid circles).