TABLE 1.
Bacterial strains and plasmids used in this study
| Strains or plasmid | Genotype and characteristics | Reference |
|---|---|---|
| S. lividans 66 | ||
| TK24 | SCP1− SCP2−a | 9 |
| G015 | SCP1− SCP2−glkA; a derivative of TK24 mutagenized by UV irradiation | This study |
| S. coelicolor A3(2) J1668 | hisA1 uraA1 strA1 pgl NF SCP2−glkΔ119; a spontaneous mutant of J1508 (10) | 6 |
| E. coli XL1-Blue | supE44 hsdR17 recA1 endA1 gyrA4 thi relA1 lac F′[proAB+ lacIqlacZ ΔM15::Tn10 (Tetr)] | 26 |
| Plasmids | ||
| pXE4 | Low-copy-number plasmid with xylE as a reporter gene | 11 |
| pEMX151 | pXE4 containing a 1.0-kb insert with the chiA promoter | 17 |
| pGA01 | pXE4 containing a 1.2-kb insert with glkA of S. coelicolor A3(2); the orientation of glkA is the same as that of xylE | This study |
| pGA02 | pXE4 containing a 1.2-kb insert with glkA of S. coelicolor A3(2); the orientation of glkA is opposite to that of xylE | This study |
| pIJ486 | High-copy-number plasmid | 9 |
| pGAH01 | pIJ486 containing a 1.2-kb insert with glkA of S. coelicolor A3(2) | This study |
| pIJ2423 | pIJ2925 (13a) containing a 1.2-kb insert with glkA of S. coelicolor A3(2) | 2 |
a
SCP2−, the strain can be introduced with pXE4.