FIGURE 2.

Imperatorin enhances SIRT1 activity and GDF15 secretion in NIH/3T3 fibroblasts. The cells were treated with imperatorin (1.5, 5, and 15 μg/mL) for 24 h. Cell lysates were harvested for examining SIRT1 activity (A), and culture supernatants were collected for measuring GDF15 secretion (B). Graphs show the mean ± SD of three independent experiments. The p-value was calculated using one-way ANOVA and multiple comparison procedures (Holm–Sidak). ^* p <0.05, ^** p < 0.01, ^*** p <0.001 compared to the control group.