Table 5. Summary of Compound 28’s Activity in TNBC Cell Lines^a.

	Cell countb		Apoptosisc
Cell line	GI50(μM)	IC50(μM)	5-Fold Induction (μM)	Emax	dG1/S cell cycle block (μM)
BT-20	0.60	0.97	7.73	6.22	0.95
BT-549	0.80	0.94	0.74	7.99	1.42
MDA-MB-231	0.80	0.88	0.64	32.4	1.78
MT-3	0.53	0.60	0.67	15.1	1.16
Hs 578T	1.06	1.23	0.78	7.24	2.13

^aBT-20, BT-549, MDA-MB-231, MT-3, and Hs 578T were treated with compound 28 in a serial dilution over 10 concentrations with a maximum of 0.1% DMSO over 72 h.

^bCell proliferation was measured by the fluorescence intensity of an incorporated nuclear dye.

^cApoptosis was measured by the fluorescence intensity of a fluorescently labeled antibody to activated caspase 3. The output is shown as a fold increase of apoptotic signal over vehicle background normalized to the relative cell count in each well. The concentration of test compound that caused a 5-fold induction in the caspase-3 signal is reported, indicating a significant apoptosis induction.

^dCell cycle arrest was measured by labeling of mitotic cells with a fluorescently labeled antibody to phosphorylated histone H3.