FIGURE 1.

Stability of transgene expression in mouse skeletal muscle. C57BL/6 male mice were injected in the left tibialis anterior with 10¹¹ GC of AAV2/8, and AAV2/rh32.33 vectors expressing the classical reporter molecules nLacZ (A), GFP (B), and ffLuc (C). At 7 and 63 days postinjection mice were sacrificed and skeletal muscle harvested for X-Gal histochemical stain (A) and immunohistochemistry (B). Live imaging was performed using a Xenogen imager to quantify luciferase expression in the skeletal muscle (C). Data are mean ± SD for four mice per group. Images represent regions of highest expression within an individual section. Statistical analysis compared AAV2/8 with AAV2/rh32.33 vectors for each time point. *, p < 0.001, by unpaired Student’s t test.