FIGURE 3.
Kinetics of T cell activation following i.m. injection of AAV2/8, and AAV2/rh32.33. C57BL/6 mice were injected with 1011 GC of AAV.nLacZ in the left tibialis anterior. At days 7, 28, and 63 postinjection, mice were sacrificed and lymphocytes harvested from spleen. IFN-γ-producing T cell responses to AAV capsid (A) and nLacZ transgene (B, primary axis) were determined by IFN-γ ELISPOT. At various time points postinjection, lymphocytes were isolated from whole blood and stained with a PE-conjugated H-2Kb-ICPMYARV tetramer together with a FITC-conjugated anti-CD8 Ab to determine the percentage of nLacZ-specific CD8+ T cells in the total CD8+ T cell population (B, secondary axis). Data are mean ± SD for four mice per group. Statistical analysis compared AAV2/8 with AAV2/rh32.33 at day 28. *, p < 0.001, by unpaired Student’s t test.