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. Author manuscript; available in PMC: 2023 Dec 18.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6051–6060. doi: 10.4049/jimmunol.0803965

FIGURE 5.

FIGURE 5.

Characterization of the T cell response to AAV.nLacZ in the presence or absence of CD4+ T cell help. C57BL/6 mice were injected with 1011 GC of AAV2/8 or AAV2/rh32.33.nLacZ in the presence or absence of the CD4+ T cell-depleting Ab, GK1.5. Lymphocytes isolated from spleen were stimulated with AAV8 and AAVrh32.33 capsid libraries (A) or the nLacZ dominant peptide (B), then evaluated by intracellular cytokine staining to measure the production of IFN-γ, TNF-α, and IL-2 cytokines by CD8+ T cells. Data are expressed as the frequency of multiple cytokine-secreting Ag-specific CD8+ T cells present 3 wk after i.m. injection. Data are mean ± SD for four mice per group. Representative data from two independent experiments are shown. Statistical analysis compared AAV2/8, AAV2/rh32.33, and AAV2/rh32.33 in combination with GK1.5 for each cytokine profile. *, p < 0.001; **, p < 0.015, by ANOVA Student-Newman-Keuls test.