FIGURE 7.

Structural comparison of the AAV8 and AAVrh32.33 capsids. A, Superimposition of the VP3 monomer of AAV8 (green) and the predicted model for AAVrh32.33 (orange). The major differences between AAV8 and AAVrh32.33 are located in variable regions I and III-VII. B, Predicted AAVrh32.33 VP3 structure (orange monomer) and the icosahedral 5- (5f), 3- (3f), and 2-fold (2f) symmetry VP monomers (all in light gray) inside gray surface density. Variable loops of AAVrh32.33 that differ from AAV8 appear as colored regions on the capsid surface: I-red, II-dark green, III-yellow, IV-green, V-cyan, VI-blue, VII-magenta, VIII-purple, IX-dark gray. The triangle defines a viral asymmetric unit bounded by 5-, 3-, and 2-fold icosahedral symmetry axes.