Figure 2.

Comparable cell proliferation in germinal matrix/corona radiata region of the subventricular zone after Intraventricular hemorrhage at postnatal day 7. (A-I) Representative immunofluorescence images labeled with Ki-67 (proliferation marker, green) and with DAPI (nucleus marker, blue). Low magnification (4×) images from GM/CR (A, D, and G) and high magnification (20×) images with Ki-67 single stain (B, E, and H) and combined Ki-67 with DAPI (C, F, and I) in the 3 experimental groups indicated at postnatal day 7. The proliferation immunofluorescence signal remained slightly reduced at day 7 in IVH + saline injected pups (middle panel: D-F) compared with no IVH healthy controls (upper panel: A-C) and a near recovery to controls in the USSC injected IVH premature pups (bottom panel: G-I). Sample size was 5 in each group, and 2-3 alternate coronal sections were taken at the level of mid-septal nucleus, 20 µm sections. Scale bar = 100 µm. Ventricle (V). (J) Quantitative distribution of total proliferating cells in the corona radiata (CR). The scatter plot with bar graph showing mean cell density ± SEM, among 3 experimental groups. Note: The total proliferating cell density in CR was comparable among 3 groups; N = 5 in each group and cells were counted on 2-3 alternate coronal sections taken at the level of mid-septal nucleus.