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. 2023 Nov;193(11):1762–1775. doi: 10.1016/j.ajpath.2023.02.002

Figure 7.

Figure 7

Western blot analysis of proteoglycans in HCFs and HKCs in three-dimensional cell culture in response to stimulation using LH 5 mIU/mL or FSH 10 mIU/mL for 4 weeks. A: Keratocan expression. HCF: control versus LH 5 mIU/mL (SEM = 0.1943); P < 0.0001. FSH 10 mIU/mL: HCF (SEM = 0.2940) versus HKC (SEM = 0.1869); P = 0.0283. LH 5 mIU/mL: HCF (SEM = 0.1943) versus HKC (SEM = 0.07302); P < 0.0001. B: Lumican expression. HCF: control versus FSH 10 mIU/mL (SEM = 0.03407); P = 0.0004. HCF: control versus LH 5 mIU/mL (SEM = 0.1350); P = 0.0073. HKC: control versus FSH 10 mIU/mL (SEM = 0.1224); P = 0.0010. HKC: control versus LH 5 mIU/mL (SEM = 0.07331); P = 0.0002. Data are expressed as means ± SEM. n = 3 for all conditions. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.