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. 2023 Dec 18;42:344. doi: 10.1186/s13046-023-02921-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Exosome miR-223-3p promotes M2 polarization of macrophages, which aggravates the proliferation and migration of CRC. A CCK-8 assays showed that proliferation of colon cancer cells was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. B The transwell assays indicated that migrative ability was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. C The colony formation assay indicated that migrative ability was significantly augmented when added the CM of THP1 cells overexpressed miR-223-3p. D A cluster heatmap of the expression profiles of cytokines in the CM of THP1 cells upon overexpression miR-223-3p or not via ELISA assays. E The expression of cytokines in THP1 cells upon overexpression miR-223-3p or not was detected by qRT-PCR. F Western blot indicated that inhibition of IL17 blocked the altered expression of E-cadherin, N-cadherin, Vimentin, Cyclin E and Cyclin D1 in colon cancer cells co-cultured with THP1 cells overexpressed miR-223-3p. G Western blot showed that the expression of IL17 was conspicuously increased or decreased in THP1 cells upon miR-223-3p overexpression or knockdown in THP1 cells, respectively. H The expression of E-cadherin, Vimentin, Cyclin E and Cyclin D1 in colon cancer cells upon administration of IL17 were detected by Western blot. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ^***P < 0.001.^**P < 0.01.^*P < 0.05. ns means the difference was not significant