FIG. 4.
Northern blot analysis of cotS mRNA. B. subtilis 168trpC2 (wild type) (A and B) and spoIIIC94 (ςK mutant; SigK−) and gerE36 (gerE mutant; GerE−) (C and D) were grown in DS medium; the cultures were harvested at the indicated times; RNA was prepared, electrophoresed, and transferred to a membrane. (A and C) 16S and 23S RNAs were visualized by staining with methylene blue. (B and D) The gene product of cotS was detected by using a digoxigenin-labeled antisense RNA of cotS as a probe. Tn shown at the top indicates the harvesting time of cells, where n is the number of hours after the end of exponential phase of growth. The arrowhead indicates the band for cotS mRNA. W.T., wild-type cell.