FIG. 5.

CotS protein in mutants lacking sporulation-specific transcription factors. The sigma factor-deficient cells and gerE mutant cells were harvested from DS medium at T₈. Whole-protein samples were solubilized from the sporulating cells and were analyzed by SDS-PAGE (12% gel) and immunoblotting using anti-ς^A (A) and anti-CotS (B) antibodies. B. subtilis 168trpC2 (lane 1), spoIIG41 (ς^E mutant) (lane 2), spoIIAC1 (ς^F mutant) (lane 3), spoIIIGΔ1 (ς^G mutant) (lane 4), spoIIIC94 (ς^K mutant) (lane 5), and gerE36 (gerE mutant) (lane 6) were analyzed. The arrowheads indicate the bands for ς^A and CotS.