FIG. 7.

Immunoblot analysis of CotS expression. B. subtilis 168trpC2 (A), 1G12 (gerE mutant) (B), and 1S105 (cotE mutant) (C) were grown in DS medium, and the cultures were harvested every hour throughout sporulation (T₀ to T₈). Whole-protein samples were solubilized from the sporulating cells and were analyzed by immunoblotting using anti-CotS and anti-ς^K antibodies. The arrowheads indicate the migration positions of ς^K and CotS.