FIG. 8.

Alignment of promoter regions of genes transcribed by ς^K RNA polymerase. (A) Sequences near the transcription start sites of genes transcribed by RNA polymerase containing ς^K. Promoters for six genes transcribed in the absence of GerE and five genes whose transcription required GerE in addition to ς^K are shown separately. Nucleotides in each promoter that match the consensus sequence are shown between the groups (m = C or A). The underlined nucleotides correspond to the transcription start point. (B) Alignment of nucleotide sequences of GerE-binding sites. The consensus sequence proposed by Zheng et al. (39) is shown at the top. Numbers refer to positions relative to the transcriptional start site. ∗, sequence from the opposite DNA strand. The bottom line shows an enlarged consensus sequence for GerE binding based on the sequence shown. R, purine; W, A or T; Y, pyrimidine. (C) Nucleotide sequence of the cotS promoter, showing putative −35 and −10 regions, the transcription start point (+1), and a ribosome-binding site (SD) (2). The boxed sequence is a putative GerE⁻ binding site in the cotS operon. References for the sequences of these promoters are as follows: sigK (spoIVCB), 20; cotA, 29; cotB and cotD, 39; cotC, 39 and 40; cotF, 9; gerE, 8; spoVK, 14; cotVXW, cotX, and cotYZ, 36; cotG, 26; and cotS, 2.