TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype or description | Source (reference) |
|---|---|---|
| B. subtilis | ||
| 168trpC2 | trpC2 | BGSCa |
| 1S101 | cotA::cat trpC2 | BGSC |
| 1S102 | cotB::cat trpC2 | BGSC |
| 1S103 | cotC::cat trpC2 | BGSC |
| 1S104 | cotD::cat trpC2 | BGSC |
| 1S105 | cotEΔ::cat trpC2 | BGSC |
| 1S106 | cotF::cat trpC2 | BGSC |
| 1S108 | cotT::pΔ E194 pheA1 trpC2 | BGSC |
| CB701 | cotS::cat trpC2 | This work |
| 1G12 | gerE36 leu-2 trpC2 | BGSC |
| 1S38 | spoIIIC94 trpC2 (ςK mutant) | BGSC |
| 1S60 | leuB8 spoIIG41 tal-1 (ςE mutant) | BGSC |
| SC1159 | spoIIAC1 (ςF mutant) | S. Cutting (10) |
| spoIIIGΔ1 | spoIIIGΔ1 trpC2 (ςG mutant) | J. Sekiguchi (31) |
| E. coli JM109 | relA supE44 endA1 hsdR17 gyrA96 mcrA mcrB+ thi Δ (lac-proAB)/F′ (traD36 proAB+ lacIqlacZΔM15) | J. Sambrook (28) |
| Plasmids | ||
| pCX18S | CmrcotS5′ lacZ′ | A. Abe (2) |
| pTUE1122 | AmprlacI tac promoter His6 | A. Nakane (23) |
| pBCS1 | AmprlacI tac promoter cotS His6 | This work |
a
BGSC, Bacillus Genetic Stock Center.