Fig. 1.

Pre-natal exposure to BPA alters chemokine gene expression. qRT-PCR shows the decreased expression of genes Cxcl2 (a), Cxcl4 (b), and Cxcl14 (d), while PCR array shows the decreased expression of genes Cxcl5 (c), Ccl8 (e), and Ccl20 (f) in female offspring. Data represent the results obtained from four treatment groups. Mice that had been exposed to BPA in utero were subsequently treated transiently with either E2 or sesame oil as vehicle control (CTL) as adults (BPA-CTL, BPA-E2; i.e, pre-natal exposure-adult treatment). Similarly, animals exposed to vehicle in utero subsequently received transient E2 or vehicle control stimulation (CTL-E2, CTL-CTL). The data are presented as relative mRNA expression in fold change relative to vehicle-treated controls (CTL-CTL). The bars in each graph represent the mean ± S.E. of three individual experiments, each performed in triplicate. *Denotes statistical significance (p < 0.05) compared to control group (CTL-CTL)