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. 2023 Dec 5;50(1):kuad044. doi: 10.1093/jimb/kuad044

Fig. 1.

Fig. 1.

Pipeline for natural product discovery by heterologous expression. Genomic DNA is sequenced and analyzed with bioinformatic tools. Biosynthetic gene clusters (BGCs) are predicted and prioritized. The selected BGCs are cloned and transferred into a suitable host for expression. Metabolomics tools are used for natural product detection, and if enough quantities are produced, the natural product is isolated and characterized. BioRender was used to generate this figure.