Table 1.
Summary of large scale, heterologous expression studies to discover natural products.
| BGC source | No. of BGCs selected for cloning | No. of BGCs cloned (success rate) | Biosynthetic class | BGC/insert size (kb) | Cloning method | Host(s) used | No. of BGCs expressed (success rate) | No. of NP families isolated | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| 1 Saccharothrix espanaensis | 25 | 17 (68%) | Multiple | 100 | Random library |
S. lividans DYA S. albus J1074 |
4 (11%) | 2 | (Gummerlich et al., 2020) |
| 14 Streptomyces spp. 3 Bacillus spp. |
43 | 43 (100%) | Multiple | 10–113 | CAPTURE |
S. avermitilis SUKA17 S. lividans TK24 B. subtilis JH642 |
7 (16%) | 5 | (Enghiad et al., 2021) |
| 100 Streptomyces spp. | Orphan PKS, NRPS, PKS-NRPS | 58 (72%) | PKS, NRPS | 140 | Random library |
S. albus J1074 S. lividans RedStrep 1.7 |
15 (24%) | 3 | (Libis et al., 2022) |
| 1 Bacteroidota 10 Pseudomonadota 3 Cyanobacteriota 5 Actinomycetota 8 Bacillota |
96 | 83 (86%) | RiPPs | <18 | Golden Gate assembly of synthetic genes | E. coli BL21 (DE3) | 27 (32%) | 3 | (Ayikpoe et al., 2022) |