TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Reference or source
Strains
E. coli AG100	Wild type	9
E. coli AG102	Mar mutant of AG100	9
E. coli BL21	Expression strain for pET vectors	Novagen
M. smegmatis mc2155	Electroporation competent	18
Plasmids
pMV261	Mycobacterium-E. coli shuttle vector	19
pPM10	pMV261::marA	This study
pPM10R	pMV261::marA in antisense orientation	This study
pPM11	pMV261::marR	This study
pPM1989R	pPM10 insertional mutant of helix A	This study (Fig. 1)
pPM2016A	pPM10 insertional mutant of helix B	This study (Fig. 1)
pET13a	T7 expression vector	20
pEC10	pET13a::marA	This study
pEC1989R	pEC10 insertional mutant of helix A	This study (Fig. 1)
pEC2016A	pEC10 insertional mutant of helix B	This study (Fig. 1)