Fig. 3.

TRAM KO resolving neutrophils mediated resolving phenotypic adaptation of leukocytes in vitro. Recipient WT neutrophils, monocytes/macrophages, and T cells were cocultured with donor cells (WT PBS control neutrophils, 4-PBA-reprogrammed neutrophils, or TRAM KO neutrophils) for 1, 1, and 4 days, respectively. Flow cytometry analysis of a the expression of CD200R and CD86 on recipient neutrophils and b the expression of CD200R on recipient monocytes/macrophages. CFSE prelabeled recipient T cells were stained for CD4 and FoxP3 expression and analyzed by flow cytometry. c The representative CFSE histogram (left) and the quantification data (right) of CD4⁺ T cells divided more than 3 times from T cell-neutrophil cocultures. d The quantification data of FoxP3⁺ Treg cell proportion in CD4⁺ T cells from cocultures. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 using one-way ANOVA test followed by the post-hoc Sidak multiple comparisons test. n = 3–5. neu neutrophils. KO TRAM KO