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. Author manuscript; available in PMC: 2023 Dec 18.
Published in final edited form as: Inflamm Res. 2023 Aug 10;72(8):1733–1744. doi: 10.1007/s00011-023-01779-z

Fig. 4.

Fig. 4

TRAM KO resolving neutrophils improved markers of endothelial integrity independent of direct intercellular interaction. Recipient endothelial cells were co-cultured with donor cells (WT or TRAM KO neutrophils) in the presence of 0.2 μM LPA for 1 day. a Flow cytometry analysis of the expression of CD31, ESAM, JAM-1, and VE-Cadherin on recipient endothelial cells from two different cocultures. In a separate experiment, endothelial cells were cultured in the neutrophil-conditioned media in the presence of LPA. The neutrophil-conditioned medium was collected from WT or TRAM KO neutrophil cultures pre-stimulated with 1 μM fMLP for 24 h. b Flow cytometry analysis of the expression of CD31, ESAM, JAM-1, and VE-Cadherin on endothelial cells cultured in two different conditioned media. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 using two-sided Student’s t-test. n = 4 or 6. neu neutrophils. KO TRAM KO