Regulation of the bfpA 5′ regulatory region deletion fusions in response to environmental cues. The activities of plasmids pCAT232, pCAT85, pCAT77, and pCAT54 were tested in EPEC strain B171-8 grown in DME medium at 37, 25, and 39°C, in LB medium at 37°C, or in DME medium containing 15 mM ammonium sulfate at 37°C or in EPEC strain T::Gmr, a bfpT mutant derived from strain B171-8 (27), grown in DME medium at 37°C. The graph shows the maximal CAT-specific activity reached late in growth. The data are representative of at least three different experiments.