FIG. 3.
Regulation of selected bfpA 5′ regulatory region mutants. The activities of bfpA-cat fusions containing down mutations (pMG51, pMG58, pMG60, and pMG65) (A) or promoter mutations (pMNC6 and pSNE10-232) (B) were tested EPEC strain B171-8 grown in DME medium at 37, 25, and 39°C, in LB medium at 37°C, or in DME medium containing 15 mM ammonium sulfate at 37°C or in strain T::Gmr, a bfpT mutant EPEC strain, grown in DME medium at 37°C. The graph shows the maximal CAT-specific activity reached late in growth. The data are representative of at least three different experiments.