FIG. 5.
Regulation of the bfpA 5′ regulatory region mutant G-12T (contained in pSNE10-232) and its 5′ deletion derivative fused to the CAT reporter gene. The activities of pSNE10-232 and its 5′ deletion derivative pSNE10-40 were tested for EPEC strain B171-10 (EPEC-10), a spontaneous plasmid-cured derivative of B171-8 (this study), or for EPEC strain T::Gmr, a bfpT mutant strain, grown in DME or LB medium at 37°C or in DME medium containing 15 mM ammonium sulfate at 37°C. For comparison, expression directed by the wild-type fusion pCAT232 in B171-10 is also shown. The graph shows the maximal CAT-specific activity reached during the culture period. The data are representative of at least three different experiments.