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Identification of ionizable lipids for potent DARPin delivery. (A) Ionizable lipid tail and core structures were reacted to form a library of 24 ionizable lipids. (B) Intracellular delivery (%GFP+ cells) of K27 by LNPs formulated with each ionizable lipid. Top performers (C12–2, C14–4, C14–5, and C16–4) are highlighted here. (C) Same library screen represented instead by the change in mean fluorescence intensity. The same top performers are again identified. n = 4.
